Determination of Glucosylated Albumin and Its Clinical Significance in Diabetes Mellitus

Manda Naoki, Nakayama Hidetaka, Aoki Shin, Sato Mitsuo, Kadota Satoru, Komori Katsutoshi, Kuroda Yoshihiko, Minakami Hirotoshi, Makita Zenji, Nakagawa Shoichi, Kudo Mamoru

doi:10.11213/tonyobyo1958.25.691

In the present study, we report the characterization of a colorimetric assay procedure for glucosylated albumin (G-A). Moreover, the level of G-A was determined in the serum of normal and diabetic subjects after purification of the albumin to apparent homogeneity.<BR>Across the range of dilutions (2.5-15 mg/m/) of the same albumin, 5-hydroxymethylfurfural (HMF) formation as determined by the thiobarbituric acid (TBA) method was a linear function of albumin concentration. Also, HMF formation from G-A during hydrolysis at 100°C with oxalic acid was progressive in the range from 1 to 4.5 hr, and then decreased slightly at 6 hr. There was a significant correlation (r=0.84) between the G-A and HbAI levels in 43 normal and 167 diabetic subjects. The correlations between the G-A and mean fasting blood sugar (FBS) levels were studied. There was a higher correlation (r=0.67) between G-A and the mean FBS within 2 weeks. On starting insulin therapy in 8 untreate juvenile diabetic subjects, there was a different temporal relationship between the FBS, G-A and HbAI levels. The G-A levels were significantly decreased at 1, 2, 3 and 4 weeks compared to the HbAI levels.<BR>The present results indicate that G-A may provide a valuable tool for assessing the mean blood sugar levels between shorter intervals, since the turnover of serum albumin is considerably faster than that of HbAI.

Determination of Glucosylated Albumin and Its Clinical Significance in Diabetes Mellitus

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