Di-(2-ethylhexyl)-phthalate reduces MyoD and myogenin expression and inhibits myogenic differentiation in C2C12 cells
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- Chen Shun-Sheng
- Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan
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- Hung Hsiao-Ting
- Department of Sports Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
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- Chen Tsan-Ju
- Department of Physiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
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- Hung Hui-Shan
- Department of Physiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
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- Wang Dean-Chuan
- Department of Sports Medicine, College of Medicine, Kaohsiung Medical University, Taiwan
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The purpose of this study was to investigate the effects of di-(2-ethylhexyl) phthalate (DEHP) treatment on MyoD and myogenin expression and myotube formation in the murine C2C12 cells. Myogenic differentiation is principally regulated by activities of myogenic regulatory factors, such as MyoD and myogenin, leading the elongation and fusion of mononucleated myoblasts into multinucleated myotubes. In the present study, myogenic differentiation of C2C12 cells was induced by serum deprivation with medium containing vehicle or DEHP (10, 100, 1,000 μg/ml) for 5 days. Using 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay clearly demonstrated cell viability was not affected by DEHP at any given dose. At the dose of 1,000 μg/ml DEHP, the elongation of multinucleated myotubes, and the percent of nuclei incorporated into myosin heavy chain (MyHC)-stained myotubes were markedly reduced. In addition, immunoblotting revealed expression of muscle specific marker MyHC, as well as myogenic regulatory factors MyoD and myogenin, were reduced in DEHP-treated myotubes during myogenic differentiation. Taken together, the results showed that DEHP may impair myogenic differentiation through repression of myogenic regulatory factors, such as MyoD and myogenin, resulting in a reduction of MyHC expression. This in vitro study suggests that DEHP may be an environmental risk factor for myogenesis.
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 38 (5), 783-791, 2013
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390001204906164736
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- NII論文ID
- 10031191737
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- NII書誌ID
- AN00002808
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- COI
- 1:CAS:528:DC%2BC3sXhslyitbrK
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 024934360
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- PubMed
- 24067726
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可