ＰＨＡマクロ法によるＨＢｓ抗体価の定量

DOI 被引用文献2件

書誌事項

タイトル別名
  • PARALLEL LINE ASSAY OF THE POTENCY OF ANTI-HBs IMMUNOGLOBULIN BY MACRO-PHA METHOD

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説明

The measurement of anti-HBs antibody potency has been carried out by RIA, PHA (Micro), CEP and Micro Ouchterlony methods. Parallel line assay by RIA method is an established method for the measurement of anti-HBs potency. We have attempted to establish a simple and precise parallel line assay method using macro-titration tubes (Macro-PHA method).<br>PHA test kit, HEBSGENCELL® was used for the Macro-PHA method. Three kind of anti-HBs immunoglobulin preparations supplyed by different distributers were diluted with phosphate buffered saline (pH 7.2) and the potencies were measured within 3 days after the dilution. Macro-PHA method was carried out in polystylene test tube (∅ 14mm, U type). The diameter of the hemagglutination ring was measured.<br>Plots of log (dilution factor) versus log (diameter) were linear between 5.5 and 10mm diameter. The slope of the three different kind of anti-HBs immunoglobulin preparations were in good parallelism stastically between one to the other. Relative potencies of the three kind of anti-HBs immunoglobulin preparations were calculated. The reproducibility of this assay was also confirmed satisfactory.

収録刊行物

  • 日本輸血学会雑誌

    日本輸血学会雑誌 27 (2), 127-129, 1981

    一般社団法人 日本輸血・細胞治療学会

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