高トリグリセリド血症検体での2種類のHDL-コレステロール直接測定法と超遠心法との比較

DOI

書誌事項

タイトル別名
  • Comparison of Two Homogeneous Methods with an Ultracentrifugation Method to Measure HDL-Cholesterol
  • Using Sera from Patients with Hypertriglyceridemia: Cross Reaction of VLDL with the Homogeneous Methods
  • 乖離要因としてのVLDLの測り込みについて

抄録

We compared the results obtained using two homegenous assays, the Daiichi method and the Kyowa method, with those obtained using an ultracentrifugation method employing sera from patients with serum triglycerides levels of 2.59 to 14.97g/l. The values obtained with the Daiichi method correlated well with those obtained with the ultracentrifugation method. Although the values obtained with the Kyowa method also correlated with those obtained with the ultracentrifugation method in most sera, significant differences were observed in four sera samples. Agarose electrophoresis showed that one of the four sera, which showed a lower HDL-cholesterol value using the Kyowa method, contained slow a HDL, and the other three, which showed higher HDL-cholesterol values using the Kyowa method, contained increased VLDL. HDL-cholesterol values of the (LDL + HDL) fractions obtained after ultracentrifugation of the three sera were similar with the two homogeneous assays. The Kyowa method, but not the Daiichi method, significantly reacted with the VLDL fractions of the three sera separated by gel filtration chromatography. None of the homogeneous methods showed abnormal reaction time courses for the three sera. These results suggest that the Kyowa method indicates higher HDL-cholesterol values in sera containing high concentrations of VLDL due to partial cross reaction with VLDL, as compared with the Daiichi method and the ultracentrifugation method.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390001204909980032
  • NII論文ID
    130003357385
  • DOI
    10.14921/jscc1971b.31.1_44
  • COI
    1:CAS:528:DC%2BD38XktFKls7w%3D
  • ISSN
    21874077
    03705633
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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