Determination of Serum Bilirubin by the Diazo Method Using the Diazotized 3-Nitroaniline Reacting Readily with the Photoproducts of Bilirubin
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- Suzuki Yuji
- Saitama College of Health
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- Sakagishi Yoshikatsu
- The first Department of Biochemistry, Saitama Medical School
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説明
We studied the diazo reaction of the water-soluble photoproducts of bilirubin using nine kinds of diazonium salts to select the diazonium salt most suitable for the determination of serum bilirubin. The diazonium salt of 3-nitroaniline was found to be more suitable than that of sulfanilic acid used extensively in clinical practice. This diazonium salt reacted readily with not only bilirubin but also the photoproducts, and the reaction of patients' serum bilirubin was completed in 2 or 3 min. The change in the measurement values obtained using this diazonium salt before and after irradiation with a fluorescent lamp was smaller than that obtained by the enzymatic method or the diazo method using the diazotized sulfanilic acid.
収録刊行物
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- Japanese Journal of Clinical Chemistry
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Japanese Journal of Clinical Chemistry 23 (2), 158-163, 1994
一般社団法人 日本臨床化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204911496320
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- NII論文ID
- 130004627113
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- COI
- 1:CAS:528:DyaK2cXmtVSjt7k%3D
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- ISSN
- 21874077
- 03705633
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可