High-molecular-mass intestinal alkaline phosphatase affects the fraction amount of bone alkaline phosphatase measured by agarose gel electrophoresis

DOI 2 Citations
  • Hoshino Tadashi
    Department of Laboratory Medicine, Nihon University School of Medicine
  • Yanai Mitsuru
    Department of Laboratory Medicine, Nihon University School of Medicine
  • Tsuchiya Tatsuyuki
    Department of Laboratory Medicine, Nihon University School of Medicine
  • Kumasaka Kazunari
    Department of Laboratory Medicine, Nihon University School of Medicine
  • Komoda Tsugikazu
    Department of Biochemistry, Faculty of Medicine, Saitama Medical University

Bibliographic Information

Other Title
  • 高分子小腸型ALPがアガロースゲル電気泳動法における骨型ALP分画値に及ぼす影響

Search this article

Abstract

We demonstrated the serum sample treated with neuraminidase and protease by agarose gel electrophoresis, and calculated the activity of bone-type alkaline phosphatase (BAP) fraction. From the result, high-molecular-mass intestinal alkaline phosphatase (HIAP) takes place to overlapping with the activity of BAP fraction. The protease-treated and non-treated samples were both subjected to agarose gel electrophoresis. Data showed a decrease in BAP in the α2β region and increase in normal-molecular-mass intestinal alkaline phosphatase in the β region were as HIAP positive. Therefore, the serum sample used for the isozyme measurement on the agalose gel electrophoresis, the protease treatment may be essential. Resulting that a good correlation (r=0.982) was found between the BAP activity determined by the mentioned-above electrophoresis method using a protease treatment, and the BAP activity determined by the ELISA method.

Journal

Citations (2)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top