書誌事項
- タイトル別名
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- Evaluation of Reactivity Using Direct Assay Methods for High Density Lipoprotein Cholesterol
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説明
We evaluated the lipoprotein specificity of two direct methods based on different principles for quantifying high-density lipoprotein cholesterol (HDL-C). Reagent a, utilizing polyethlene glycol-modified enzymes and sulfated α-cyclodextrin, showed about 6% crossreactivity for very low-density lipoprotein (VLDL), while reagent b which consisted of polyanion and dispersive surfactant showed about 5% crossreactivity for low-density lipoprotein (LDL). There was difference in the reactivity for HDL3 among the two direct methods and the precipitation method, but both direct methods exhibited a higher cholesterol value for HDL2 than the precipitation method. To investigate the reactivity to HDL2 in detail, the HDL2 fraction was separated into HDL with apo E and HDL without apo E by heparin-sepharose affinity chromatography. The precipitation method measured only HDL without apo E, but HDL-C measured by the two direct methods included both of HDL with and without apo E. HDL-C values by the direct method were in agreement with the values of total cholesterol in HDL fractions isolated by ultracentrifugation.
収録刊行物
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- Japanese Journal of Clinical Chemistry
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Japanese Journal of Clinical Chemistry 26 (3), 150-156, 1997
一般社団法人 日本臨床化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204912276480
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- NII論文ID
- 130003357832
- 10011508905
- 10003205942
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- NII書誌ID
- AN00252758
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- COI
- 1:CAS:528:DyaK2sXmslant78%3D
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- ISSN
- 21874077
- 03705633
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可