Proposal for “Fundamental principles of cell culture”

DOI
  • Kanda Yasunari
    Division of Pharmacology, National Institute of Health Sciences
  • Nakamura Kazuaki
    Department of Pharmacology, National Research Institute for Child Health and Development
  • Yamazaki Daiju
    Division of Pharmacology, National Institute of Health Sciences
  • Kataoka Ken
    Department of Life Science, Faculty of Science, Okayama University of Science
  • Aoi Takashi
    Division of Advanced Medical Science, Graduate School of Science, Technology and Innovation, and Department of iPS Cell Applications, Graduate School of Medicine, Kobe University
  • Nakagawa Masato
    Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University
  • Fujii Makiko
    International Collaboration Development for Dentistry, School of Density, Hiroshima University
  • Akutsu Hidenori
    Department of Reproductive Biology, National Research Institute for Child Health and Development
  • Suemori Hirofumi
    Laboratory of Embryonic Stem Cell Research, Institute for Frontier Life and Medical Sciences, Kyoto University
  • Asaka Isao
    Department of Fundamental Cell Technology, Center for iPS Cell Research and Application (CiRA), Kyoto University
  • Nakamura Yukio
    Cell Engineering Division, RIKEN BioResource Center
  • Kojima Hajime
    Division of Risk Assessment, National Institute of Health Sciences
  • Sekino Yuko
    Division of Pharmacology, National Institute of Health Sciences Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Furue Miho K
    Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition

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Other Title
  • 「細胞培養における基本原則」の提案

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Abstract

<p>Recently, cell culture technique has evolved rapidly into an effectively and widely used research tool for basic cell science, pharmaceutical study and regenerative medicine. In Europe and the United States, the Guidance on Good Cell Culture Practice (GCCP) has been proposed to promote the maintenance of high standards and to reduce uncertainty in the development and application of animal and human cell and tissue culture procedures and products. GCCP encourages greater international harmonization, rationalization and standardization of laboratory practices, quality control systems, safety procedures, recording and reporting, and compliance with laws, regulations and ethical principles. In Japan, with an aim of sharing the basic concepts for cell culture by researchers, we drew a draft guidance, “Fundamental principles of cell culture”. This guidance consists of five sections; vulnerability of cultured cells, credibility of cell source and validity of culture method, prevention of microorganism- and cross- contamination, documentation, handling safety and environmental protection. We propose here the guidance as common principles for researchers who are using or interact with cell culture in Japan.</p>

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