A Novel Mixed Valence Form of Rhus vernicifera Laccase and Its Reaction with Dioxygen to Give a Peroxide Intermediate Bound to the Trinuclear Center.
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- Zoppellaro Giorgio
- Department of Chemistry, Faculty of Science, Kanazawa University
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- Sakurai Takeshi
- Department of Chemistry, Faculty of Science, Kanazawa University
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- Huang Hong-wei
- Department of Chemistry, Faculty of Science, Kanazawa University
書誌事項
- タイトル別名
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- Novel Mixed Valence Form of Rhus vernicifera Laccase and Its Reaction with Dioxygen to Give a Peroxide Intermediate Bound to the Trinuclear Center
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抄録
Rhus vernicifera laccase, in a novel mixed valence state [T1oxT23red: type 1 Cu as Cu(II), and type 2 and 3 Cus as Cu(I)], was formed by reacting Cu(I) on the type 2 Cudepleted lactase [T1oxT3red: type 1 Cu as Cu(II) and type 3 Cus as Cu(I)] under argon. Contrary to T1oxT3red, T1oxT23red was highly reactive with dioxygen, and gave the three transient bands at 340, 475, and 680nm due to the two-electron reduced form of dioxygen [charge transfer bands from peroxide to Cu(II)]. The first order decays were highly dependent on pH, which led to the successful detection of the intermediate for ca. 2h at pH 7.5. Another mixed valence derivative, T12oxT3red [type 1 and type 2 Cus as Cu(II), and type 3 Cus as Cu(I)] prepared through the action of Cu(II) on T1oxT3red was not reactive with dioxygen, but showed high enzyme activity as to the oxidation of N, N-dimethyl-p-phenylenediamine. The whole reaction mechanism of the reduction of dioxygen by lactase was proposed based on the present results together with data for the former detection and characterization of the three-electron reduced form of dioxygen [Huang, H. et al. (1999) J. Biol. Chem. 274, 46, 32718-32724].
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 129 (6), 949-953, 2001
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204930537728
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- NII論文ID
- 130003534167
- 10007856419
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD3MXls1SgsLY%3D
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 5785913
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- PubMed
- 11388911
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可