Expression of -N-Acetylgalactosaminylated N-Linked Sugar Chains Is Associated with Functional Differentiation of Bovine Mammary Gland
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- Sato Takeshi
- Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology
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- Taka Junko
- Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology Institute of Natural Sciences, Faculty of Education, Saitama University
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- Aoki Naohito
- Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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- Matsuda Tsukasa
- Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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- Furukawa Kiyoshi
- Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology
書誌事項
- タイトル別名
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- Expression of .BETA.-N-Acetylgalactosaminylated N-Linked Sugar Chains Is Associated with Functional Differentiation of Bovine Mammary Gland.
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説明
During lactation the mammary gland synthesizes a large amount of glycoproteins including those composing milk fat globule membrane (MFGM). Our previous study showed that N-linked sugar chains with Ga1NAcβ1→4GIcNAc structure appears to increase in bovine MFGM glycoproteins during early lactation [Ujita et al. (1993) FEBS Lett. 332, 119-122]. Western blot analysis of membrane glycoproteins from lactating and post-lactating bovine mammary glands using Wistaria floribunda agglutinin (WFA), which binds oligosaccharides terminating with β-N-acetylgalactosamine, and Ricinus communis agglutinin-I (RCA-I), which binds oligosaccharides preferentially terminating with β-1, 4-galactose, showed that the number and reactivity of protein bands to WFA but not to RCA-1 decrease drastically in the post-lactating mammary sample. Establishment of primary cultured epithelial cells from lactating bovine mammary gland and their culture on collagen-coated dishes in the presence of a mixture of lactogenic hormones revealed that N-linked sugar chains with Ga1NAcβ1→4GlcNAc structure are expressed in the functionally differentiated cells without altering the apparent β-galactosylation of the oligosaccharides. These results strongly suggest that the expression of GalNAcβ1→4GlcNAc structure on N-linked sugar chains is associated with the mammary gland differentiation.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 122 (5), 1068-1073, 1997
社団法人 日本生化学会