A novel human metalloprotease synthesized in the liver and secreted into the blood: possibly, the von Willebrand factor‐cleaving protease?
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- Soejima Kenji
- First Research Department
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- Mimura Noriko
- First Research Department
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- Hirashima Masaki
- First Research Department
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- Maeda Hiroaki
- First Research Department
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- Hamamoto Takayoshi
- Blood Products Research Department, The Chemo-Sero-Therapeutic Research Institute
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- Nakagaki Tomohiro
- Blood Products Research Department, The Chemo-Sero-Therapeutic Research Institute
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- Nozaki Chikateru
- First Research Department
書誌事項
- タイトル別名
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- A Novel Human Metalloprotease Synthesized in the Liver and Secreted into the Blood: Possibly, the von Willebrand Factor-Cleaving Protease?
- Novel Human Metalloprotease Synthesized in the Liver and Secreted into the Blood Possibly the von Willebrand Factor Cleaving Protease
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We identified a novel metalloprotease, which could be responsible for cleaving the Tyr842-Met843 peptide bond of von Willebrand factor (vWF). This metalloprotease was purified from Cohn Fraction-I precipitate of human pooled plasma by the combination of gel filtration, DEAE chromatography, and preparative polyacrylamide gel electrophoresis in the presence of SDS. The NH2-terminal amino acid sequence of the isolated protein was: AAGGILHLELLVAVGPDVFQAHQEDTRRY. Based on this sequence, we searched human genomic and EST databases, and identified compatible nucleotide sequences. These results suggested that this protein is a novel metalloprotease, a member of the family of a disintegrin and metalloprotease with thrombospondin type-1 motifs (ADAMTS), and its genomic DNA was mapped to human chromosome 9q34. Multi-ple human tissue northern blotting analysis indicated that the mRNA encoding this protease spanned approximately 5 kilobases and was uniquely expressed in the liver. Furthermore, we determined the cDNA sequence encoding this protease, and found that this protease was comprised of a signal peptide, a proregion followed by the putative furin cleavage site, a reprolysin-type zinc-metalloprotease domain, a disintegrin-like domain, a thrombospondin type-1 (TSP1) motif, a cysteine-rich region, a spacer domain, and COOH-terminal TSP1 motif repeats.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 130 (4), 475-480, 2001
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204932054272
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- NII論文ID
- 130003534261
- 10007858884
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD3MXovVCqtr0%3D
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- ISSN
- 17562651
- 0021924X
- http://id.crossref.org/issn/0021924X
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- NDL書誌ID
- 5993138
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- PubMed
- 11574066
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可