Genetic and morphological differences of Haematobia irritans and H. exigua, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)

IWASA Mitsuhiro, ISHIGURO Naotaka

doi:10.7601/mez.61.335

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Other Title

mtDNA塩基配列に基づいたノサシバエHaematobia irritans とH. exigua の遺伝的，形態的差異および日本産サシバエ類の分子系統(双翅目：イエバエ科，サシバエ族)
Genetic and morphological differences of Haematobia irritans and H exigua and molecular phylogeny of Japanese Stomoxyini flies Diptera Muscidae

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Molecular analysis based on mitochondrial DNA (mtDNA) sequence was performed to elucidate the genetic difference between Haematobia irritans (Linnaeus) and H. exigua (de Meijere), and the phylogenetic relationship among Japanese Stomoxyini species (Stomoxys calcitrans (Linnaeus), S. indicus Picard, S. uruma Shinonaga et Kano, and Haematobosca sanguinolenta (Austen) including Haematobia). Two populations obtained from Obihiro and Morioka which were morphologically identified as H. irritans were genetically identical. Two populations obtained from Taiwan and Vietnam which were morphologically identified as H. exigua were almost genetically identical, but there was a small divergence (0.3-0.4%) between them. Nucleotide sequence divergences between H. irritans from Obihiro-Morioka and H. exigua from Taiwan-Vietnam were 1.8-1.9%, and those between Haematobia and other separate species of Stomoxys and Haematobosca were 6.7-10.9%. In cluster analysis based on mtDNA, Haematobia species were close to Haematobosca sanguinolenta. S. calcitrans and S. uruma were closely related to each other, and S. indicus was distantly related to these two species. We discussed the status of H. irritans and H. exigua based on mtDNA, and the discrepancies of molecular phylogeny versus the previous hypotheses based on morphological criteria in Japanese Stomoxyini.

Journal

Medical Entomology and Zoology

Medical Entomology and Zoology 61 (4), 335-344, 2010

The Japan Society of Medical Entomology and Zoology

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Genetic and morphological differences of <i>Haematobia irritans</i> and <i>H. exigua</i>, and molecular phylogeny of Japanese Stomoxyini flies (Diptera, Muscidae)