An artificial blood feeding system for body louse, Pediculus humanus

  • KASAI Shinji
    Department of Medical Entomology, National Institute of Infectious Diseases
  • MIHARA Minoru
    Department of Environmental Biology, Japan Environmental Sanitation Center
  • TAKAHASHI Masakazu
    Department of Medical Entomology, National Institute of Infectious Diseases
  • AGUI Noriaki
    Department of Medical Entomology, National Institute of Infectious Diseases
  • TOMITA Takashi
    Department of Medical Entomology, National Institute of Infectious Diseases

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  • コロモジラミの人工吸血法の研究(英文)
  • artificial blood feeding system for body louse Pediculus humanus

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Abstract

Human body louse is a significant vedor of infectious diseases including typhus, relapsing fever and trench fever. In order to perform an infection assay of these pathogens efficiently using human louse, and to maintain colonies of louse in the laboratory without human volunteers, we attempted to establish an artificial blood feeding system. Using parafilm^[○!R] membranes and blood-absorbed cotton, we made a blood pad and body lice were fed on the pad fixed on a petri-dish. As a result, body lice efficiently engorged blood through the membrane and more than 30 percent of the hatched larvae reached the adult stage. This system was suitable for performing an artificial infection assay although it was still insufficient for maintaining a colony for many generations.

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