Influence of Corticosteroids on Coagulation Factors: Study of Patients with Immune Thrombocytopenia and Human Liver Cancer HepG2 Cells

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  • Mori Mika
    Department of Pediatrics, St. Marianna University School of Medicine, Yokohama City Seibu Hospital, Kanagawa, Japan
  • Akita Mieko
    Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa Japan
  • Umezawa Yotaro
    Department of Pediatrics, St. Marianna University School of Medicine, Yokohama City Seibu Hospital, Kanagawa, Japan
  • Ashikaga Tomoko
    Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa Japan
  • Yamashita Atsuki
    Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa Japan
  • Nagae Chiai
    Department of Pediatrics, St. Marianna University School of Medicine, Kanagawa Japan
  • Yamazaki Satoshi
    Department of Clinical Laboratory, St. Marianna University School of Medicine Hospital, Kanagawa Japan
  • Takayama Shigenobu
    Faculty of Health Science, Daito Bunka University, Tokyo, Japan
  • Kaneko Hanae
    Department of Radioisotope Research Institute, St. Marianna University School of Medicine, Kanagawa, Japan
  • Nawa Yukino
    Department of Radioisotope Research Institute, St. Marianna University School of Medicine, Kanagawa, Japan
  • Matsui Hiroaki
    Department of Radioisotope Research Institute, St. Marianna University School of Medicine, Kanagawa, Japan
  • Taki Masashi
    Department of Pediatrics, St. Marianna University School of Medicine, Yokohama City Seibu Hospital, Kanagawa, Japan

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  • 副腎皮質ステロイドの凝血学的影響：免疫性血小板減少症およびヒト肝癌細胞株HepG2細胞における検討

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Abstract

<p>Background: The relation between corticosteroids and thrombosis continues to remain uncertain. We investigated the effects of methylprednisolone (m-PSL) treatment on coagulation factors in patients before and after pulse steroid therapy for immune thrombocytopenia (ITP) and studied the m-PSL-induced changes in mRNA expression of various coagulation factor genes using HepG2 cells.<br/>Materials and Methods: m-PSL (30 mg/kg/dose) was administered intravenously for 3 days to patients with ITP (n=3). The changes in fibrinogen levels and activities of prothrombin, FV, FVII, FVIII, FIX, FX, FXI, and FXII before treatment and one day after the end of treatment were analyzed. Furthermore, the mRNA expression of coagulation factor genes in the cultured HepG2 cell treated with m-PSL (100μM) was also analyzed by RT-PCR.<br/>Results: An increase in FVIII: C (p=.00064) was observed in the patients with ITP after the m-PSL pulse therapy. However, in the experiments in which cultured HepG2 cells were treated with m-PSL, there was no increase in the expression of the coagulation factor genes, including the FVIII gene. However, the expression of FXI mRNA alone was decreased significantly (p=.044).<br/>Discussion: The results of the increased FVIII: C after m-PSL treatment suggested the induction of the hypercoagulable state and supported finding of previous reports. However, the relation of the coagulation factors between the activity changes of the patients with ITP and the mRNA expression in HepG2 cells was inconsistent. Further investigations to clarify the mechanism underlying the hypercoagulable state after m-PSL treatment is necessary.</p>

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