Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector
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- Itaya Mitsuhiro
- Mitsubishi Kagaku Institute of Life Sciences
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- Fujita Kyoko
- Mitsubishi Kagaku Institute of Life Sciences
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- Ikeuchi Masahiko
- Department of Life Sciences (Biology), University of Tokyo
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- Koizumi Maki
- Mitsubishi Kagaku Institute of Life Sciences
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- Tsuge Kenji
- Mitsubishi Kagaku Institute of Life Sciences
書誌事項
- タイトル別名
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- Stable Positional Cloning of Long Continuous DNA in the <i>Bacillus subtilis</i> Genome Vector
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説明
Direct cloning of a long continuous genome segment in a Bacillus subtilis genome vector was demonstrated for the first time. Two small DNA fragments had to be installed in the vector prior to cloning. The DNA between these two fragments was cloned via homologous recombination. The efficiency of cloning was estimated using the 3, 573-kb genome of a cyanobacterium, Synechocystis sp. PCC 6803. Recombinants were selected using the internal selection system of the Bacillus genome vector or with the antibiotic resistance marker in the cyanobacterial genome. Designated genomic segments as large as 77-kb were cloned by means of a single procedure. Cloning efficiency is affected by the molecular weight of the donor DNA and the size of the DNA to be cloned. The method is suitable for direct target cloning of large-sized DNA.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 134 (4), 513-519, 2003
社団法人 日本生化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204964427648
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- NII論文ID
- 130003534594
- 10012058225
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- NII書誌ID
- AA00694073
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- COI
- 1:CAS:528:DC%2BD3sXhtVWgtbrN
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- ISSN
- 17562651
- 0021924X
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- NDL書誌ID
- 6732796
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- PubMed
- 14607977
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可