Studies on the "Transfer Factor" of Tuberculin Hypersensitivity : II.Passive transfer of tuberculin hypersensitivity with fractions of either disrupted alveolar macrophages or serum of sensitized and challenged rabbits

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  • Izumi Takateru
    The Second Department of Internal Medicine, Tuberculosis Research Institute, Kyoto University

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Other Title
  • ツベルクリン感受性伝達因子に関する研究 : 第2篇 ウサギのAlveolar Macrophages及びその細胞画分並びに血清画分による「ツベルクリン」感受性の受身伝達
  • ツベルクリン感受性伝達因子に関する研究-2-
  • ツベルクリン カンジュセイ デンタツ インシ ニ カンスル ケンキュウ 2

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Since the first success of Chase in 1945, it has been thought in general that the passive transfer of tucerculin hypersensitivity is successful when viable lymphoid cells of sensitive donor animals are inoculated into recipients and that if non-viable cellular constituents of these cells are used, passive transfer of sensitivity is almost impossible. Neverthless, the writer performed passive transfer experiments using either alveolar macrophages which are new materials for passive transfer experiments or the serum of sensitized and challenged rabbits. Results are as follows. (1)It was shown that living alveolar macrophages have apparent capacity to recipients tuberculin sensitive by intraperitoneal inoculations of 10'or more cells. (2)In passive transfer experiments with cellular fractions separated by successive centrifigations of alveolar macrophage disrupted by five cycles of freezing and thawing, mitochondria fraction seemed to have a definite capacity to confer sensivity. It was of note that cellular supernatant (after centrifigation by 20,000 G 90 minutes) per se had no activity to confer sensitivity, but afetrt dialysis against distilled water, transfer activity appeared in this fraction. It may be postulated that a certain low molecular factor possessing activity to inhibit transfer of tuberculin hypersensitivity(Inhibitor) is contained in dialysed outer fluid of the cellular supernatant. Nuclei and microsomes had no transfer activity. (3)Dialysed inner fluids of sera of sensitized and challenged rabbits showed apparent activity to confer sensitivity, though serum per se had no activity. Thus, it was very likely that also serum contained both transfer factor and inhibitor. (4)Tuberculin hypersensitivity transfered passively developed between the first and the third day after administration of test materials and disappeard on or after the seventh day. It may be due to two treatments, challenge of animals and separation of inhibitor from test materials, that passive transfer of tuberculin hypersensitivity with non viable materials are constantly sucessful despite many unsuccesful previous investigation.

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