Nephrotoxic Serum Antigen Isolated from Rat Aorta

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  • Kitamura Kiyoshi
    The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo

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  • 大動脈壁に含まれるネフロトキシン有効抗原
  • ダイドウミャクヘキ ニ フクマレル ネフロトキシン ユウコウ コウゲン

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It is well known that the active antigen that induces nephrotoxic antibody is located not only in the kidney (the glomerular basement membrane) but also in various other organs (especially in lung and aorta), and that the antigen is extracted in the form of water-so'uble substance using tryptic digestion. In this paper, nephrotixic serum antigen isolated from rat aortic wall was studeid by enzymatic digestion, gel filtration, zone electrophoresis and fluorescent antiboky technique, and the following findings were obtained: 1) The antigenic activity of four ultrasupernatant samples prepared after digestion by four different enzymes was studeid; the order of antigenic activity was as follows: collagenase->tryptic->elastase->pronase-digested ultrasupernatant sample. 2) Through enzymatic digestion, gel filtration and zone electrophoretic studies, it was demonstrated that the composition of this antigen has the character of glycoprotein, same as that of the glomerular basement membrane. 3) Immunoflurescent studies of rat aortic wall revealed that the nephrotoxic serum antigen is mainly lacated, in the form of intercellular substance, in tunica media.

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