Precipitating Antibodies to Mite Antigen Detected in Asthmatic Patients

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  • Ito Koji
    Department of Internal Medicine and Physical Therapy, School of Medicine, University of Tokyo

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  • 気管支喘息患者に検出されたダニに対する沈降抗体
  • キカンシ ゼンソク カンジャ ニ ケンシュツサレタ ダニ ニ タイスル チンコ

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In order to determine the antibody activity to mites, the mite antigen was labelled with radioactive iodine. In the course of the studies, the precipitating antibodies were detected in the sera of allergic patients. The results were presented in this paper. The mite antigen extracted from cultured mites (Dermatophagoides farinae) was labelled with ^<125>I. Sera from patients allergic to mite and house dust were examined in double gel diffusion test using this labelled antigen. The positive reaction was observed in 16 of 37 patients. No reaction was observed in healthy individuals so far as the author examined. The intensity of the precipitating line did not correlate to the titer of IgE antibodies determined by RAST. Radioimmunoelectrophoresis was carried out with these sera and labelled mite antigen. IgG antibodies were detected in these sera. IgE antibodies were also detected in these sera by radioimmunodiffusion. From these results antibodies which belong to these class of immunoglobulins seem to be related to the precipitation reaction. Although IgA and IgM antibodies were not detected with these technique so far as the author examined, presence of these antibodies can not be denied, because the sensitivity of these techniques might be still low for the detection. For the exact determination of antibody activity which belongs to each class of immunoglobulin, the double antibody method should be applied.

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