INFLUENCE OF ANTIGEN/ANTIBODY BINDING RATIOS IN DNA-ANTI-DNA COMPLEXES ON DNA BINDING RADIOIMMUNOASSAY USING DNase

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  • Kazuta Yasuhito
    Third Department of Internal Medicine, Kinki University School of Medicine
  • Ikeda Keizo
    Third Department of Internal Medicine, Kinki University School of Medicine
  • Hashimoto Kiyoyasu
    Third Department of Internal Medicine, Kinki University School of Medicine
  • Horiuchi Atsushi
    Third Department of Internal Medicine, Kinki University School of Medicine

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  • DNA分解酵素を用いたDNA-抗DNA免疫複合体測定法の検討
  • DNA ブンカイ コウソ オ モチイタ DNA コウ DNA メンエキ フクゴ

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Abstract

Following two problems were indicated in the measurement of DNA-anti-DNA complexes by DNA binding radioimmunoassay (RIA) using DNase I. 1)The use of high-valency-DNA as antigen in the DNA binding RIA resulted in low sensitivity of the assay, and that made the values of DNA-anti-DNA complexes measured by DNA binding RIA less than those measured by radioallergosorbent test (RAST). 2)The DNA, bound with anti-DNA antibody in zone of antibody excess, was demonstrated hardly digestible with DNase I. The DNA-anti-DNA complexes in SLE sera were considered to be formed in zone of antibody excess, and that resulted in insufficient digestion with DNase I. In conclusion, DNA binding RIA using DNase I was proved inadequate to the measurement of DNA-anti-DNA complexes in SLE sera.

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