STUDIES ON ALLERGENS OF "MAIKO" IN "KONNYAKU ASTHMA" : ISOLATION AND IDENTIFICATION OF ANTIGENICITY

DOI Web Site
  • Furukawa Mitsuru
    The First Department of Internal Medicine, School of Medicine, Gunma University
  • Nakazawa Tsugio
    The First Department of Internal Medicine, School of Medicine, Gunma University
  • Kobayashi Setsuo
    The First Department of Internal Medicine, School of Medicine, Gunma University
  • Sato Kumiko
    Department of Paracytology, School of Medicine, Gunma University

Bibliographic Information

Other Title
  • こんにやく喘息の抗原に関する研究 : 第1編 15% Polyacrylamide Gel Electrophoresis による精製
  • こんにゃく喘息の抗原に関する研究-1-15%Polyacrylamide Gel Electrophoresisによる精製
  • コンニャク ゼンソク ノ コウゲン ニカンスルケンキュウ 1 15パーセント

Search this article

Abstract

"Konnyaku asthma", primarily indigenious to Japan, is an occupational disease caused by inhalation of a dust called "Maiko". The dust is a by-product of the milling of the tuberous roots of Amorphophalus konjac, used to produce "konnyaku", a grey, jelly-like foodstuff, much used by the Japanese people. We have already reported that Ag40, an antigen precipitated from the water soluble fraction of "Maiko" by 40% saturated ammonium sulfate, had the most potent reactivity against the reagin of konnyaku asthmatic patients. In this work, Ag40 was further fractionated using 15% polyacrylamide gel electrophoresis into four fractions. One of these, designated Ag40D-2, elicited the most intensive reaction in the PK test. Reelectrophoresis of Ag40D-2 showed one band which stained with Amido black 10B and slightly stained with PAS. SDS-polyacrylamide gel electrophoresis of Ag40D-2 revealed the molecular weight of the antigen to be about 24 thousand. Ag40D-2 was composed of sixteen amino acids. Half-cystine, tryptophan and hexosamine, however, were not detected in it. The ratio of acidic amino acids to basic amino acids was 3.7 to 1.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top