Induction of Immunological Tolerance to Rabbit Gamma Globulin in Adult Mice : Influence of Anti-Mouse Immunoglobulin Antibodies on Induction of Tolerance

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  • 成熟マウスのウサギγグロブリンに対する免疫学的寛容の誘導 : 抗マウス免疫グロブリン抗体の寛容成立におよぼす影響
  • セイジュク マウス ノ ウサギガンマ グロブリン ニ タイスル メンエキガクテ

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Abstract

Immunological tolerance against RGG was induced easily in adult C3H mice by the administration of normal RGG deaggregated by ultracentrifugation. But it could hardly be induced by the administration of deaggregated RGG containing small dose of anti-mouse Ig antibody (ab-RGG), and the threshold of tolerance against ab-RGG was high. The same was observed by the administration of deaggregated RGG containing small dose of anti-mouse serum albumin antibody. To elucidate this fact further, 1 mg of deaggregated RGG containing various amount of specifically purified anti-mouse Ig antibody was used as tolerogen. Though the tolerance occured in all animals by the RGG containing less than 10 μg of antibody, it did not occur in about half of animals by the RGG containing 25 μg of antibody. The tolerance was not induced in almost all of animals by the RGG containing 100 μg of antibody. That is, when the amount of antibody contained in RGG increased, the rate of animals became tolerant decreased. The tolerance occured easily by the deaggregated ab-RGG in animals pretreated by suitable amount of carrageenan which was known to impede the function of macrophage. From these results, it was concluded that the induction of tolerance was inhibited by the presence of antibody against host component regardless of its specificity. So, it was suggested that the immune complex produced in vivo and phagocytosis of it by macrophage played a important role in determining whether tolerance was induced or not.

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