CROSS-REACTIONS OF CERTAIN HUMAN ANTINUCLEAR ANTIBODIES WITH HUMAN IgG

DOI Web Site 1 Citations
  • Shibata Takanori
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Soeda Keiko
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Takase Shigeru
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Saito Kenichi
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Kawasumi Hisashi
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Uchida Jun
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Kitazawa Kozo
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Yonekura Masahiro
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Shiwachi Shigeyo
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Kan Keiji
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Sugisaki Tetsuzo
    The Dirst Department of Internal Medicine, Showa University, School of Medicine
  • Ito Shogo
    Department of Pathology, SUNY at Buffalo N.Y.U.S.A.

Bibliographic Information

Other Title
  • 抗核抗体のヒトIgG (特にFc部分) との交叉反応性について
  • コウカク コウタイ ノ ヒト IgG トクニ Fc ブブン ト ノ コウサ ハ

Search this article

Abstract

Activity of antinuclear antibody (ANA) in sera from patients with systemic lupus erythematosus (SLE)(16 cases), rheumatoid arthritis (RA)(3), mixed connective tissue disease (MCTD)(2), progressive systemic sclerosis (PSS)(1), membranous nephropathy (MN)(1), Hashimoto's thyroiditis (1) and autoimmune hemolytic anemia (1) were studied following incubation with IgG of various species including human, rabbit and rat by means of indirect immunofluorescence. The disappearance or the abolishment of ANA activity after incubation of the sera with IgG of human origin, but not of the other species origin, was observed in 10 of 16 cases of SLE, in one of three cases of RA, in two of two cases of MCTD and in one of each PSS and MN. The binding site of human IgG to ANA was studied by incubation of the sera with pepsin treated-IgG, PEG treated-IgG, S-sulfonated-IgG, and IgG Fc fragment. The activity of ANA disappeared or abolished after incubation with PEG treated- and S-sulfonated-IgG, but not with pepsin treated-IgG, indicating that Fc portion of IgG had an antigenicity to ANA. Moreover, precipitation against heat aggregated human IgG (or Fc) by double immunodiffusion method was observed in two (or three) of five cases of rheumatoid factor negative SLE sera which contained cross-reactive ANA. These results could suggest that human ANA has reactivity with human IgG, especially Fc portion and that certain ANA (mainly IgG ANA) has also an activity as rheumatoid factor.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top