DIFFERENCE BETWEEN MITE ANTEGENS (DERMATOPHAGOIDES PTERONYSSINUS) BOUND TO IgE OF ALLERGIC PATIENTS AND THOSE BOUND TO IgE OF IMMUNIZED MICE

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  • Abe Tatsuya
    Department of Parasitology, Miyazaki Medical College
  • Ishii Akira
    Department of Parasitology, Miyazaki Medical College

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  • ダニアレルギー患者と免疫マウスのIgEに結合するヤケヒョウヒダニ抗原の差違について

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The posibility of producing murine IgE with allergens of Dermatophagoides pteronyssinus (D.p.) was studied. The fraction most reactive to a pooled serum from eight mite-allergic patients (Fr.IIB) was collected from D.p. body-extract by gel-filtration on a Sephacryl S-200 column and injected to produce IgE antibody into BALB/c mice with aluminum hydroxide. The IgE-binding activities of components in Fr.IIB were measured by ELISA and PCA for human and murine sera, respectively. Sephadex G-75 column chromatography and isoelectric focusing were used to detect differences between the components bound to human and to murine IgE antibodies. The components producing the murine IgE were isoelectrically and serologically different from the allergens which were bound to human IgE. In spite of the fact that the fraction injected into the mice was allergen-rich, no murine IgE antibody against the allergens was detected in the serum.

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