THEOPHYLLINE REDUCES SERUM LEVELS OF ECP IN VITRO
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- Shibahara Shuichi
- Clinical Research Laboratory, Mie National Hospital
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- Fujisawa Takao
- Department of Pediatrics, Mie National Hospital
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- Terada Akihiko
- Department of Pediatrics, Mie National Hospital
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- Iguchi Kosei
- Department of Pediatrics, Mie National Hospital
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- Kato Yoshiko
- University of Mie Graduate School of Medicine
Bibliographic Information
- Other Title
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- Eosinophil Cationic Protein(ECP)の血清中濃度測定値に及ぼすテオフィリンの影響
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Abstract
Measurement of serum levels of ECP has been widely used for monitoring airway inflammation in bronchial asthma and recently been applied to measure anti-inflammatory effect of theophylline. However, reduced levels of ECP in thephylline-administered patients may express not only in vivo effect of theophylline but also in vitro effect after sampling because serum ECP measures released ECP during coagulation and theophylline has been reported to inhibit eosinophil degranulation in vitro. In order to answer the question, we tested whether theophylline added to blood after sampling reduces measured levels of serum ECP. Various concentrations of theophylline were added to SST^【○!R】 tube, to which venous blood from atopic patients was drawn. Serum was, then obtained by centrifugation after 15 min to 6hours of incubation at room temperature. Theophylline significantly reduced serum ECP in a concentration-dependent manner. Percent reduction of ECP levels at 1 hour of incubation were 11.9%, 18.7%, 22.8%, and 51.7% at theophylline levels of 5, 12.5, 22.5, and 120 μg/ml, respectively. Kinetics of serum ECP release was also inhibites in the presence of theophylline. These results suggest that in vitro effect of theophylline on serum ECP levels should be considered when data of serum ECP in patients who take theophylline are interpreted.
Journal
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- Japanese Journal of Allergology
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Japanese Journal of Allergology 47 (5), 518-523, 1998
Japanese Society of Allergology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204981050880
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- NII Article ID
- 110002404832
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- NII Book ID
- AN00012583
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- ISSN
- 13477935
- 00214884
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed