EFFECT OF OSMOLALITY ON ION TRANSPORT IN GUINEA PIG AIRWAY EPITHELIAL CELLS

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  • Hattori Tomohiro
    Division of Respiratory Medicine, Department of Medicine, Nihon University School of Medicine
  • Majima Toru
    International University of Health and Welfare, Chemotherapy Institute, Kaken Hospital, Respiratory Center
  • Hashimoto Shu
    Division of Respiratory Medicine, Department of Medicine, Nihon University School of Medicine

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Other Title
  • 浸透圧変化によるモルモット気道上皮イオントランスポートの反応性の検討
  • シントウアツ ヘンカ ニ ヨル モルモット キドウ ジョウヒ イオントランスポート ノ ハンノウセイ ノ ケントウ

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Abstract

Background: Increased osmolality of the airway surface fluid due to water loss associated with hyperventilation is considered to be a cause of exercise-induced asthma (EIA). Aim: We investigated the influence of changes in osmolality on airway epithelial ion transport in guinea pigs. Methods: We explored from measuring open circuit potential difference in challenge with hypertonic saline or mannitol. Results: Diphenylamine-2-carboxylate (DPC), amiloride, and disodium cromoglycate (DSCG) prevented an increase of the potential difference (PD) after exposure of tracheal mucosa to 0.9-10.8% hypertonic saline solutions (HSSs) (p<0.05, p<0.01, and p<0.01, respectively on 1.8%). An increase of the PD was observed after a single dose of 1.8% hypertonic saline solution (HSS) was applied to the mucosa, but no increase was observed after a single exposure to a 585mOsm/kg aqueous mannitol solution (585AMS). The results remained the same when a Cl-free solution was used as the perfusate. The change of airway epithelial cell thickness was only suppressed significantly by DPC (p<0.01) after 1.8% HSS challenge. Conclusions: Not only the change of osmolality, but also changes of the Cl^- and Na^+ concentrations in airway surface fluid seem to have an important influence on the PD. cAMP-dependent Cl^- channel may have a role, and the same mechanism may provoke EIA.

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