COMPARISON OF USEFULNESS BETWEEN VARIABLE NUMBERS OF TANDEM REPEATS (VNTR) ANALYSIS AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) IN THE GENOTYPING OF MYCOBACTERIUM AVIUM

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  • KAZUMI Yuko
    Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA)
  • UDAGAWA Tadashi
    Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA)
  • MAEDA Shinji
    Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA)
  • MURASE Yoshirou
    Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA)
  • SUGAWARA Isamu
    Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA)
  • OKUMURA Masao
    Respiratory Medicine
  • AZUMA Yuka
    Medical Laboratory, Fukujuji Hospital, JATA
  • GOTO Mieko
    Department of Infection Control and Prevention, The University of Tokyo Hospital
  • TSUNEMATSU Noriko
    Department of Clinical Laboratory, Tokyo Metropolitan Otsuka General Hospital

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  • Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ａｖｉｕｍタイピングにおけるＶａｒｉａｂｌｅ　Ｎｕｍｂｅｒｓ　ｏｆ　Ｔａｎｄｅｍ　Ｒｅｐｅａｔｓ（ＶＮＴＲ）法とＲｅｓｔｒｉｃｔｉｏｎ　Ｆｒａｇｍｅｎｔ　Ｌｅｎｇｔｈ　Ｐｏｌｙｍｏｒｐｈｉｓｍ（ＲＦＬＰ）法の有用性の比較
  • Mycobacterium avium タイピング ニ オケル Variable Numbers of Tandem Repeats VNTRホウ ト Restriction Fragment Length Polymorphism RFLPホウ ノ ユウヨウセイ ノ ヒカク

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Abstract

[Objectives] Comparison of usefulness of ISI245RFLP and VNTR in M. avium genotyping.<BR>[Materials and methods] Th irty-six cases (55 strains) from sputum and BALF and twelve cases (29 strains) isolated from blood of HIV-infected patients were used. VNTR and RFLP using ISI245 were performed.<BR>[Result] Multipl e samples were taken from 16 patients and 52 clinical isolates were used for VNTR and RFLP for comparison. (1) VNTR and RFLP results were identical in 12out of 16 cases whose samples were collected several times. (2)Eight isolates were obtained from one patient. In this eight isolates, there were the cases of M. avium polyclonal infection and of mixed infection with M. intracellulare. VNTR patterns were two types and RFLP were 5 kinds of different in this case. (3) VNTR patterns of six isolates from one H1V-infected patient were identical, but there were three variations in RFLP patterns.<BR>There were three cases of mixed infections with M. tuberculosis or M. intracellulare, and six strains polyclonal infection of M. avium (7.1%) in 84 isolates. These 6 clinical isolates were derived from sputum or BALF (5 strains) and HIVinfected blood (one strain).<BR>VNTR patterns w ere similar in four pairs (9 strains) who did not contact closely, but they were distinguished clearly by RFLP. Seventeen strains had three or less ISI245-related bands in RFLP analyses of 89 strains.<BR>[Discussion] As there is a possibility of polyclonal infection with M. avium and mixed infection with other species, the single clonal infection should be confirmed first by VNTR. When single colony was obtained, VNTR and RFLP were performed for genotyping of M. avium. Furthermore, strains with less bands by RFLP should be carefully judged in terms of both VNTR and RFLP. It is recommended that the specimens should be collected from each patient several times.

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