Serological Diagnosis of Pulmonary Tuberculosis and Nontuberculous Pulmonary Mycobacteriosis.
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- KONDO Ariyoshi
- Niigata Tetsudo-Kenshin Center, East Japan Railway Company
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- OKETANI Norihiro
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- MARUYAMA Michio
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- SAITO Yasuharu
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- MIYAO Hiromi
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- OHNO Michiko
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- WADA Koichi
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- TSUCHIYA Toshimasa
- Department of Respiratory Medicine, National Nishi-Niigata-Chuo Hospital
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- NAGAI Tadashi
- Department of Clinical Laboratory, National Nishi-Niigata-Chuo Hospital
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- TSUCHIYA Kuniko
- Department of Clinical Laboratory, National Nishi-Niigata-Chuo Hospital
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- WATANABE Yasushi
- Department of Clinical Laboratory, National Nishi-Niigata-Chuo Hospital
Bibliographic Information
- Other Title
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- 肺結核症ならびに非結核性肺抗酸菌症の血清診断
- ハイケッカクショウ ナラビニ ヒケッカクセイ ハイ コウサンキンショウ ノ ケッセイ シンダン
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Description
Objective: The present study was undertaken to evaluate the utility of the serodiagnosis of pulmonary tuberculosis and nontuberculous pulmonary mycobacteriosis by ELISA using a Pathozyme-Myco kit (Myco kit) and a Pathozyme-TB complex kit (TB kit) (OMEGA Diagnostics Ltd.).<BR>Study population: The subjects comprised 256 healthy volunteers (HV, healthy hospital employees), 66 patients with sputum-positive active pulmonary tuberculosis (apTB), 14 patients with healed pulmonary tuberculosis (hp TB), 24 patients with nontuberculous pulmonary mycobacteriosis (NTM) and 32 patients with pulmonary diseases other than mycobacteriosis.<BR>Results: 1) The serum IgG antibody titers determined with the Myco kit were significantly higher in the apTB group (p<0.01), the hp TB group (p<0.01), and the NTM group (p<0.01) than those in the HV and the other pulmonary disease group. At a cut-off value of the mean +2SD of the values obtained in the HV, the positive rate was 47.0% in patients with apTB, 50.0% in those with NTM, 21.4% in those with hp TB, 3.1% in those with other pulmonary diseases, and 1.6% in the HV. Analysis of ROC curves showed that the HV and the pulmonary mycobacteriosis group (apTB and NTM) were best distinguished by a cut-off value of -0.280 OD (log), with the sensitivity and the specificity being 83.3% and 78.5%, respectively. It was impossible to distinguish apTB from NTM. 2) The serum IgG antibody titers determined with the TB kit were significantly higher in the apTB group than those in the HV (p<0.01), the NTM group (p<0.05) and the other pulmonary disease group (p<0.01). No significant difference was observed between the HV and the patients with NTM or those with other pulmonary diseases. Although the positive rate of the test was low in the apTB group (42.4%), there was a significant difference between apTB and NTM (12.5%) (p<0.05), suggesting that apTB could be distinguished from NTM. 3) Since the serum antibody titers determined by the Myco kit showed no significant difference between apTB and NTM, and there was also no difference in the positivity between the two diseases, we performed serologic examination using the Myco kit to detect both diseases as pulmonary mycobacteriosis. After diagnosing pulmonary mycobacteriosis by the Myco kit, we then used the TB kit to separate apTB from NTM. In this case, the sensitivity and specificity of the test were 55.6% and 85.7%, respectively. Better methods should be developed to distinguish apTB from nontuberculous mycobacteriosis.
Journal
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- Kekkaku(Tuberculosis)
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Kekkaku(Tuberculosis) 76 (9), 603-614, 2001
JAPANESE SOCIETY FOR TUBERCULOSIS
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Keywords
Details 詳細情報について
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- CRID
- 1390001204999606912
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- NII Article ID
- 10007716818
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- NII Book ID
- AN00073442
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- ISSN
- 18842410
- 00229776
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- NDL BIB ID
- 026778782
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- PubMed
- 11676117
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed