COMPARISON OF MACCONKEY'S AGAR MEDIUM AND HYDROXYLAMINE MEDIUM FOR DIFFERENTIATION OF <I>MYCOBACTERIUM FORTUITUM</I> COMPLEX FROM OTHER RAPIDLY GROWING MYCOBACTERIA

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  • Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ｆｏｒｔｕｉｔｕｍ　Ｃｏｍｐｌｅｘを他の迅速発育性抗酸菌から区別する方法としてのＭａｃＣｏｎｋｅｙ寒天培地とＮＨ２ＯＨ培地の比較
  • Mycobacterium fortuitum Complex オ タ ノ ジ

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Abstract

MacConkey's agar medium and hydroxylamine egg medium were compared for differentiating strains of the Mycobacterium fortuitum complex from other rapidly growing mycobacteria.<BR>Only 40% of the strains of M. fortuitum and one half of the strains of M chelonei subsp. abscessus could grow on the MacConky's agar plates or slants, although the procedure described in the Manual of Clinical Microbiology (1974) has been used faithfully. In addition, about one half of the strains of M. smegmatis grew on this medium.<BR>In contrast, all strains of the M. fortuitum complex grew on the hydroxylamine medium (0.5 mg/ml NH2OH·HCl). However, one half of the strains of M. smegmatis and all strains of M. chitae grew on this medium. On the hydroxylamine medium (0.75 mg/ml), 80% of the strains of M. fortuitum, 92% of the strains of M. chelonei subsp. abscessus could grow, while about 50% of the strains of M. chelonei subsp. chelonei failed to grow on it. All other strains did not grow on this medium.<BR>The hydroxylamine medium (0.5 mg/ml and 0.75 mg/ml) appeared to be more suitable for differentiating the strains of the M. fortuitum complex from other rapidly growing mycobacteria than did the MacConkey's agar. As stated previously by the present authors (7), it is required to use a combination set of three or more tests to make a complete differentiation.

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