Relationship between Clinical Indicators and the Total Amount of Bacteria in the Tongue Coat Assessed by Real-time PCR in Oral Care Evaluation

KISHI Mitsuo, TAKAHASHI Masahiro, KISHI Kayo, HAREYAMA Fumiko, TAMURA Kohei, ABE Akiko, SUGIURA Go, AIZAWA Fumie, YONEMITSU Masami

doi:10.5834/jdh.56.5_665

We assessed the relationship between clinical and bacteriological indicators on the efficacy of oral care. The subjects comprised 27 patients of a rehabilitation hospital in Iwate prefecture. We evaluated the oral status of the subjects clinically via inspection of the tongue coat volume (tongue coat score), CPI, and measurement of oral malodor (VSC: H_2S and CH_3SH levels). Subsequently, tongue coat samples were collected from the subjects for biological assessment. These samples were weighed and applied to real-time PCR to quantify the total bacterial cells. Examinations were performed both on admission and two months after admission. At two months after admission, clinical indicators were improved, especially the tongue coat score, CPI, and CH_3SH level. For the bacteriological indicators, the tongue coat volume was reduced significantly and the mean number of bacterial cells in the whole tongue coat sample tended to decrease. However, the mean number of bacterial cells per 1mg of tongue coat was almost the same. A correlation was observed between the H_2S level and the number of bacterial cells per 1mg of tongue coat. In step-wise multiple regression models in which the dependent variable was the number of bacterial cells in the whole tongue coat, the tongue coat score and H_2S level were both significant variables. In conclusion, assessment of the tongue coat volume and oral malodor via the H_2S level were efficient clinical indicators to deduce bacteriological changes after oral care.

Relationship between Clinical Indicators and the Total Amount of Bacteria in the Tongue Coat Assessed by Real-time PCR in Oral Care Evaluation

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