DIFFERENTIATION OF NOCARDIA AND MYCOBACTERIA BY THE ACIDFAST STAINING OF S M EARS

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  • 抗酸染色によるNocardiaとMycobacteriaの鑑別
  • コウサン センショク ニ ヨル Nocardia ト Mycobacteria ノ カンベツ

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Abstract

Differentiation of Nocardia from Mycobacteria is generally not difficult according to the cultural properties. Sometimes, however, it is not easy to differentiate them, especially in the microscopic examination of sputum specimens, owing to the acidfast stain of the fragments of some of the Nocardia.<BR>As report e d previously, analytical studies carried out on the decolorization procedures of the acidfast staining led us to the finding that within 20 minutes rinsing in a decolorizer composed of O.1-0.5% HNO3 or HC1 in 70% (v/v) Ethanol resulted in the complete loss of the stain of saprophytic Mycobacteria. In the present studies, two kinds of acid-ethanol, namely, 0.1% HNO3-70% Ethanol and 4% HNO3-Ethanol, were used for the decolorization.<BR>The results revealed that the former was very effective in decolorizing Nocardia almost completely by less than 30 seconds' rinsing, by which time Mycobacteria retained nearly 50%of stain. On the contrary, by the use of the latter, the commonly used decolorizer, such a marked difference was not observed between Nocardia and Mycobacteria.<BR>Based on the results obtained, a very simple method was devised to differentiate Nocardia from Mycobacteria by the acidfast stain of smears employing 0.1% HNO3-70% Ethanol as a decolorizer for 10 to 30 seconds.

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