EFFECT OF TWEEN 80 ON THE TUBERCULIN REACTION

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  • ASAMI Nozomu
    From Department of Tuberculosis, National Institute of Health, Tokyo, Japan.
  • KATAOKA Tetsuro
    From Department of Tuberculosis, National Institute of Health, Tokyo, Japan.

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Other Title
  • ツベルクリン反応に及ぼすTween80添加の影響
  • ツベルクリン ハンノウ ニ オヨボス Tween80 テンカ ノ エイキョウ 1 カンサ ドウブツ ニ オケル Tween80 テンカ PPDs ニ ヨル ハンノウ
  • 1. Tuberculin Reaction in Guinea Pigs Caused by the PPDs Added with Tween 80
  • 1.感作動物におけるTween80添加PPDsによる反応

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Abstract

PPD solution in world wide use by WHO, RT 23, is added with Tween 80 to protect the drop of its potency. The present study was carried out to know the effect of Tween 80 addition on the tuberculin reaction.<BR>Samples tested wer e PPDs solution in phosphate buffer and phosphate buffer with or without addition of Tween 80 in a concentration of 0.005%, respectively. The permeability of blood vessels was checked employing Voisin's method. Namely, Evans blue solution was injected intravenously at the destined time intervals from 0 to 48 hours after intradermal injection of the samples, and the size of spot appeared at the site of intradermal injection was measured.<BR>Results: 1) Difference was observed in the effect of Tween 80 according to the difference of lot or the manufacturing date, and the older the Tween 80, the more marked was the modification of the tuberculin reaction.<BR>2) The relative potenc y of PPDs added with Tween 80 was 3 to 4 times stronger than that without addition of Tween 80, and the reaction caused by the former was remarkably larger than that of the latter. Above mentioned modifying effect was more pronounced in the case where tuberculin reaction was weak.<BR>3) Tween 80 resulted in the ob v ious increase in the permeability of blood vessels from the start to 30 minutes after the intradermal injection.<BR>From these results the addition of Tween 80 to PPDs seemed to be unfavorable to expect accurate diagnosis of tuberculosis infection by tuberculin.

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