STUDIES ON THIACETAZONE RESISTANCE OF TUBERCLE BACILLI

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  • 結核菌のThiacetazone耐性に関する研究
  • ケッカクキン ノ Thiacetazone タイセイ ニ カンスル ケンキュウ 1 ミチリョウ ショウニ ケッカク カンジャ ヨリ ブンリ シタ ケッカクキン ノ Thiacetazone カンジュセイ
  • Part I: Thiacetazone Sensitivity of Tubercle Bacilli Isolated from Previously Untreated Tuberculous Children
  • 第1編未治療小児結核患者より分離した結核菌のThiacetazone感受性

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Many workers reported the thiacetazone sensitivity of tubercle bacilli isolated from patients in different countries, such as England, South India, East Africa and Hong Kong. These reports showed that British and East African strains were more sensitive than South Indian and Hong Kong strains to thiacetazone.<BR>This paper presents th e sensitivity to thiacetazone of tubercle bacilli isolated from the previously untreated tuberculous children in Japan. The strains were stored in refrigerator after the lyophilization. The ampule containing 5 mg of tubercle bacilli was opened and 5 ml of sterile distilled water was poured in to get 1 mg, ml bacillary suspension.0.2 ml of 1 mg/ml suspension was inoculated into two Tween Albumin Dubos media and incubated for 10 to 14 days for getting 0.18 to 0.2 density by spectrophotometer. The diluted cultures were inoculated 0.1 ml each on the two slopes of 1 Ogawa's medium conaining 0, 0.25, 0.5, 1, 2, 4 and 8 mcg/ml thiacetazone. The inoculum size was 10-3 and 10-5 mg. The reading was made at 2, 4 and 6 weeks in the 10-3 mg inoculation and at 4 and 6 weeks in the 10--5mg inoculation.<BR>The sensitivity to thiacetazone was tested in 40 strains. The results are shown in Tables 1 and 2. Among 40 strains, 11 strains (27.5%) showed 0.5 mcg/ml or less MIC and 20 strains (50%) showed 1 mcg ml MIC at 4 weeks' reading in the 10-3 mg inoculation.9 strains (22.5%) showed 2 mcg/ml or more MIC. Geometric mean MIC of 40 strains was 0.966 and the value was situated between the value of British, East African and that of South Indian, Hong Kong strains.<BR>The proportion of resistant mutants on thiacetazone containing media was widely different by the strains (Table 2).<BR>Above mentioned te s t of the sensitivity to thiacetazone was performed by the use of Tibione made in Japan. The comparison of drug activity for tubercle bacilli between Conteben made in Germany and Tibione made in Japan was done to make sure the activity of Tibione. As seen in Table 3. the activity of both drugs was not different.<BR>In conclusion, the tubercle bacilli isolated from th e previously untreated tuberculous children in Japan showed moderate thiacetazone resistance,

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