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- 中島 眞樹
- 京都大学結核胸部疾患研究所胸部外科 滋賀医科大学第2外科 滋賀医科大学第2外科
書誌事項
- タイトル別名
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- Morphological Study of Monocrotaline-induced Pulmonary Hypertension in Rats
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説明
A single dose of 50mg/kg Monocrotaline was injected i.p. in male S-D rats aged 5-6 weeks. Pulmonary hypertension manifested in almost all rats 4 weeks after injection of monocrotaline. The systolic and mean pressures of the pulmonary artery were 57mmHg and 38mmHg respectively. The weights of right ventricles and the wall thickness of the pulmonary trunk in monocrotaline-treated rats were significantly greater than those of normal rats.<br>Morphological changes due to this agent were seen in the pulmonary alveoli and pulmonary vascular system. Pulmonary vascular changes: The changes in pulmonary vasculature of monocrotaline-treated rats included medial thickness and perivascular edema of pulmonary artery, vasoconstriction, muscularisation of arteriole, vascular obstruction and arteritis. Ultrastructurally, changes in endothelial cells of the artery were particularly noticeable. Vesicles and mitochondria increased in the cytoplasm of endothelial cells. Cell nuclei were sometimes significantly enlarged with scanty chromatin which was suggestive of loss of endothelial function. Other than these findings, these cells had various patterns, they were almost normal, showed apparent cyst formation in the cytoplasm which compressed the nuclei, had vacuolation, contained intensely heterotypic nuclei, revealed a tendency to cell destruction, or contained abundantly Weibel-Palade bodies. All of these findings seemed to be directly related with the increased vascular permeability.<br>Alveolar changes: Histological examination of the alveoli revealed the pulmonary edema, intra-alveolar haemorrhage, proliferation of alveolar cells and multiplication of mast cells. Ultrastructurally the proliferation of type B alveolar epithelial cells increased in monocrotaline-treated rats compared to those in normal rats, suggesting slightly larger size of individual cells with abundant lamellar inclusion bodies. Vacuoles were also seen in the cytoplasm of these cells, in addition to frequent evidence of marked mitochondrial development. Ultrastructurally vacuoles and micropinocytotic vesicles increased in general in the endothelial cells of the capillary. Mast cells were rarely seen in the alveolar septa of normal rats, but proliferation of mast cells rich in granules having strong affinity for osmium was remarkable in the alveolar septa in monocrotaline-treated rats.<br>These findings were also believed to be involved greatly in the increased capillary permeability and occurrence of alveolar interstitial edema.
収録刊行物
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- 日本胸部疾患学会雑誌
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日本胸部疾患学会雑誌 21 (3), 227-239, 1983
社団法人 日本呼吸器学会
