Quantification of Very Low Density Lipoprotein Apo C and Apo E Peptides by Isoelectric Focusing in Type IV Hyperlipidemia, Familial Chylomicronemia and Xanthelasma Palpebrae without Hyperlipidemia

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  • Wada Kazunari
    Department of Internal Medicine, Yamaguchi University School of Medicine
  • Miki Hideo
    Department of Internal Medicine, Yamaguchi University School of Medicine
  • Okuda Fumio
    Department of Internal Medicine, Yamaguchi University School of Medicine
  • Kusukawa Reizo
    Department of Internal Medicine, Yamaguchi University School of Medicine

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  • ヒトＶｅｒｙ　Ｌｏｗ　Ｄｅｎｓｉｔｙ　Ｌｉｐｏｐｒｏｔｅｉｎ　ａｐｏ　Ｃおよびａｐｏ　Ｅ　ｐｅｐｔｉｄｅｓの定量と，Ｆａｍｉｌｉａｌ　ＣｈｙｌｏｍｉｃｒｏｎｅｍｉａならびにＸａｎｔｈｅｌａｓｍａ　Ｐａｌｐｅｂｒａｅにみられる特徴
  • ヒト Very Low Density Lipoprotein apo C オ

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Abstract

Human very low density lipoprotein (VLDL) apoprotein peptides have been quantified by analytical isoelectric focusing.<br>Material and Method<br>The subjects are 20 healthy human, 7 patients with type IV hyperlipidemia, 3 patients of xanthelasma palpebrae without hyperlipidemia and 3 of familial chylomicronemias. For isoelectric focusing, polyacrylamide gel (7.3%, 8×0.7cm) containing 8M Urea and 1.63% Ampholine pH 3.5-7, was used.<br>Results<br>A good lineality of densitometric response was obtained between concentrations of 50 and 150μg of VLDL total protein per gel. As for reproducibility, coefficient of variation was less than 7.4%.<br>Apo E variant, E-IV, was observed in 2 of 3 familial chylomicronemias and one of 7 type IV hyperlipidemias. No apo E-IV was observed in 20 healthy subjects.<br>In healthy subjects, apo E-II/E-III ratio was 0.48 (Mean). The mean values of type IV hyperlipidemia and familial chylomicronemia are 0.46 and 0.56, respectively. While, the ratio was higher, 0.61, in the patients of xanthelasma palpebrae without hyperlipidemia than in the others.<br>Relative decrease of VLDL apo E-III in those patients of xanthelasma may be associated with the heterozygous form of type III hyperlipidemia as reported by Douste-Blazy et al. VLDL apo E-IV may have a role of inhibition of lipoprotein lipase-activity in the patients with hypertriglyceridemia like as familial chylomicronemia.

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