高比重リポ蛋白 (HDL) の臨床的意義に関する研究 (第1報)

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タイトル別名
  • High-Density Lipoprotein Cholesterol Determination by Three Different Methods
  • コウ カジュウ リポ タンパク HDL ノ リンショウテキ イギ ニカンスルケ
  • HDLコレステロール測定法の検討

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An inverse relationship has been demonstrated between plasma high-density lipoprotein (HDL) cholesterol level and the incidence of coronary heart disease. Therefore, measurement of HDL cholesterol is of great importance.<br>In the present study we compared three different methods for the estimation of HDL cholesterol in plasma samples.<br>The methods were as follows.<br>(1) Ultracentrifugation.<br>Cholesterol was determined in the d<1.063g/ml fraction from 2ml of plasma after centrifugation at 100, 000G for 20hr.<br>(2) Heparin-Mn2+ precipitation.<br>To 2ml of plasma we added 80μl of a heparin solution (5000units/ml) and 100μl of MnCl2 solution (1Mol/l), and immediately mixed. After the sample had stood for 30min. in an ice bath, the sample was centrifuged (1500G, 30min., 4°C) and cholesterol was determined in the supernate.<br>(3) Sodium phosphotungstate-Mg2+ precipitation.<br>To 2ml of plasma we added 200μl of a sodium phosphotungstate solution and 50μl of MgCl2 solution (2Mol/l). After mixing in a vortex mixer, the sample was centrifuged (1500G, 30min., 4°C) and cholesterol was determined in the supernate.<br>HDL cholesterol levels of 138 subjects were measured by two precipitation methods and for 32 of them the three methods were used simultaneously.<br>The correlation coefficients were 0.939 between ultracentrifugation and heparin-Mn2+ precipitation methods, 0.919 between ultracentrifugation and phosphotungstate-Mg2+ methods, and 0.966 between the two precipitation methods.<br>Our results showed similar results for the cholesterol content of HDL determined by the three methods.<br>Both precipitation methods are simple and appropriate for routine clinical laboratory use.

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