Investigation on the Determination Method of Total Iron Binding Capacity Using Radioactive Iron

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  • OKAZAKI Toru
    Central clinical laboratory, Shiohama Hospital, Mie Prefectural University School of Medicine
  • OKADA Hiroaki
    Central clinical laboratory, Shiohama Hospital, Mie Prefectural University School of Medicine
  • KATO Tamio
    Central clinical laboratory, Shiohama Hospital, Mie Prefectural University School of Medicine
  • YANAGIDA Makoto
    The third department of Internal Medicine, Mie Prefectural University School of Medicine
  • MIYAMURA Satoru
    The third department of Internal Medicine, Mie Prefectural University School of Medicine

Bibliographic Information

Other Title
  • 放射性鉄を用いて血清総鉄結合能を測定する方法の検討
  • 放射性鉄を用いて血清総鉄結合能を測定する方法の検討--RES-O-Mat Fe Kit「TIBC」の検討
  • ホウシャセイ テツ オ モチイテ ケッセイ ソウ テツ ケツゴウノウ オ ソク
  • (RES-O-Mat Fe Kit[TIBC]の検討)
  • (A Method Using Res-O-Mat Fe Kit [TIBC])

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Description

Determination of Total Iron Binding Capacity (TIBC) using Res-O-Mat Fe kit [TIBC] offered by Daiichi Radioisotope Laboratories, Ltd. was investigated. This kit method is to determine the total iron binding capacity of transferrin by counting the radioactivity of 59Fe which was bound to transferrin after removing the serum iron from the transferrin by adding 0.6% citric acid. About 95% of unbounded 59Fe was adsorbed by Resin Strip (Amberlite IRA 400) after 90 minutes incubation, and this method is very simple and sufficient for clinical use. TIBC value determined by this method well agreed with the value of (UIBC+SI) determined by the other method. Denaturation of transferrin due to change of serum pH was investigated by Ouchterlony's method and immunoelectrophoresis, but no change of transferrin was observed. Tf value determined by immunodiffusion method showed fairly good correlation with TIBC value. SI value determined by colorimetry also well agreed with the value of (TIBC-UIBC). This kit method is found to be simple and useful in clinical application.

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 15 (12), 1303-1308, 1974

    The Japanese Society of Hematology

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