書誌事項
- タイトル別名
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- A Simple Analytic Method of Activation Mechanism of Factor IX Using Western Blotting
- 部分純化物を用いたWestern Blotting法による第9因子活性化機序の簡易解析法
- ブブン ジュンカブツ オ モチイタ Western Blottingホウ ニ
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抄録
The proteolytic activation of partially purified F.IX induced by F.XIa/Ca2+ was investigated in the patients with hemophilia B variants. When partially purified normal F.IX (BaCl2 adsorption and elution) was cleaved by F.XIa/Ca2+, a polypeptide band (MW=60,000) disappeared with the concordant appearance of a peptide with MW=50,000 (F.IXaβ) on SDS gel autoradiography in the absence of reducing agent. In the presence of reducing agent, a new band with MW=30,000 (heavy chain of F.IX) was observed. F.IX (Niigata) and F.IX (Kiryu) with Hemophilia BM were cleaved by F.XIa/Ca2+ at the rate similar to normal F.IX. F.IX (Takatsuki) with Hemophilia BM was slowly cleaved by F.XIa/Ca2+ compared to normal F.IX. When F.IX (Kashihara) was activated by F.XIa/Ca2+, no new band was seen in non-reducing gels, whereas only one new band appeared in reducing gels, corresponding to MW=40,000. These showed that F.IX (Kashihara) was cleaved only at Arg-Ala bond and not at Arg-Val bond. F.IX (Nagoya I), F.IX (Nagoya II) and F.IX (R) were not cleaved by F.XIa/Ca2+ in this system. The proteolytic activation of partially purified F.IX induced by F.XIa/Ca2+ was investigated in the patients with Hemophilia B variants using western blotting technique.
収録刊行物
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- 臨床血液
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臨床血液 28 (1), 47-52, 1987
一般社団法人 日本血液学会
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詳細情報 詳細情報について
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- CRID
- 1390001205031755648
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- NII論文ID
- 130004918492
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- NII書誌ID
- AN00252940
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- COI
- 1:STN:280:BiiC1Mbis1E%3D
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- ISSN
- 18820824
- 04851439
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- NDL書誌ID
- 3123770
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- PubMed
- 3573331
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可