The Measurement of β-Thromboglobulin by Enzyme Immunoassay Technique

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  • TANAKA Masao
    Hematological Disease Center, Nagoya National Hospital
  • HIROTA Yutaka
    Hematological Disease Center, Nagoya National Hospital
  • KATO Kanefusa
    Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony

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Other Title
  • β-トロンボグロブリンのエンザイムイムノアッセイによる測定法の開発
  • ベータ トロンボ グロブリン ノ エンザイム イムノアッセイ ニヨル ソクテイ

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Abstract

Sensitive enzyme immunoassay system for measurement of β-thromboglobulin (β-TG) in plasma was prepared with purified antibody to β-TG and β-D-galactosidase from Escherichia coli as labeled substance. The antibodies were purified with an immunoaffinity chromatography form the antiserum, which had been produced in rabbits by injecting purified β-TG. The assay system consisted of the polystyrene bead with immobilized antibody F (ab')2 fragments and the antibody Fab' fragments labeled with β-D-galactosidase. The assay was highly sensitive with β-TG measurable at levels as low as 10 pg/assay tube. β-TG levels in plasma was measured with 2.5 μl samples. Under the conditions, coefficient of variation for within and between run was less than 8% and the results obtained with the enzyme immunoassay correlated well with those with a radioimmunoassay. The plasma β-TG levels in healthy subjects determined with the present assay system were 38.4±6.2 ng/ml and the levels were markedly high in patients with myeloproliferative syndrome and in patients with DIC.

Journal

  • Rinsho Ketsueki

    Rinsho Ketsueki 23 (2), 150-154, 1982

    The Japanese Society of Hematology

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