好中球のNitroblue-Tetrazolium (NBT)還元能に関する研究

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書誌事項

タイトル別名
  • Study on Nitroblue-Tetrazolium (NBT) Reduction by Neutrophils
  • 好中球のNitroblue-Tetrazolium(NBT)還元能に関する研究-1-組織化学的半定量化の試み
  • コウチュウキュウ ノ Nitroblue Tetrazolium NBT カン
  • I. 組織化学的半定量化の試み
  • I. A New Histochemical Method to Detect NBT Reduction by Neutrophils

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抄録

NBT test, introduced by Park et al in 1968, has been widely used as a rapid and simple aid to detect chronic granulomatous disease (CGD) and systemic bacterial infections. However, technical problems chiefly due to NBT-heparin complex were not solved. Recently the author found that NBT dye itself had anticoagulant activity and was able to establish a new histochemical method without using heparin.<br>In this semi-quantitative method neutrophils are classified into five types (O, I, II, III and IV) on the basis of the pattern of formazan deposit in the cytoplasm. Cells of each type are given a square of the type number for their degree of positivity. (O=0, I=1, II=22 (4), III=32 (9), IV=42 (16)) The NBT score is defined as the sum of the degree of 100 cells and NBT rate as the percentage of positive cells.<br>Normal controls showed mean NBT score 101.5 (O-2.5%, I-96.3%, II-1.1%, III-0.1%, IV-0%) and mean NBT rate 96.5%.<br>Most patients having systemic bacterial infections showed elevated scores over 200 and positive cells of III and IV were increased in number. Patients having non-bacterial diseases did not show high score except two cases of SLE with slightly elevated scores.<br>In two cases of CGD, normal scores were obtained even in severe pseudomonas infection. Their neutrophils, however, completely failed to respond to endotoxin and scores remained within normal range.<br>Principles of the method and technical procedures are chiefly mentioned in this report.

収録刊行物

  • 臨床血液

    臨床血液 17 (12), 1266-1275, 1976

    一般社団法人 日本血液学会

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