Upgrading of Flow Cytometric Analysis for Absolute Counts, Cytokines and Other Antigenic Molecules of Cynomolgus Monkeys(Macaca fascicularis) by Using Anti-Human Cross-Reactive Antibodies.

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  • YOSHINO Naoto
    AIDS Research Center, National Institute of Infectious Diseases Faculty of Industrial Science and Technology, Science University of Tokyo
  • AMI Yasushi
    Division of Experimental Animal Research, National Institute of Infectious Diseases
  • TERAO Keiji
    Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases
  • TASHIRO Fumio
    Faculty of Industrial Science and Technology, Science University of Tokyo
  • HONDA Mitsuo
    AIDS Research Center, National Institute of Infectious Diseases

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In order to effectively use cynomolgus monkeys as animal models for human diseases, more than 300 anti-human monoclonal antibodies (mAbs) were studied as to their cross-reaction with various antigens from cynomolgus monkeys (Macaca fascicularis). Two hundred twenty-nine of 339 (67.55%) anti-human mAbs that react with human antigens of CD-defined molecules, chemokine receptors, and T cell receptors were cross-reactive with the monkey antigens. Using the cross-reactive antibodies and the fluorescenced beads for calibration, the procedure for the absolute count of monkey lymphocyte subsets was developed and the mean values for CD4+ and CD8+ lymphocyte subsets in peripheral blood were 718 and 573/mm3, respectively. Moreover, intracellular cytokines, IL-2, IL-4 and IFNγ, and intracellular apoptosis-related proteins, Bcl-2, FADD and active form of caspase-3 could be detected in peripheral blood mononuclear cells as well as various tissue cells. It is therefore practicable to detail the phenotype of leukocytes, assess the production of intracellular cytokines and enumerate T-lymphocyte subsets by using the cross-reactive human antibodies with respective antigens of cynomolgus monkeys.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 49 (2), 97-110, 2000

    公益社団法人 日本実験動物学会

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