Rederivation of Mice by Means of In Vitro Fertilization and Embryo Transfer.

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  • SUZUKI Hiroshi
    Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
  • YOROZU Keigo
    Chugai Pharmaceutical Co., Ltd.
  • WATANABE Toshihiko
    Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
  • NAKURA Masao
    Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.
  • ADACHI Jiro
    Laboratory of Molecular Genetics and Embryology, CSK Research Park INC.

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  • Rederivation of Mice by Means of In Vit

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Abstract

In vitro fertilization and embryo transfer were performed for rederivation of four strains of mice harbouring mouse hepatitis virus (MHV) and/or Pasteurella pneumotropica (P. pneumotropica). Superovulated oocytes were fertilized by preincubated cauda epididymis sperm in vitro. Fertilized eggs at 2-cell stage were transferred into the oviducts of specific pathogen free (SPF) recipients. Microbial examination of sperm and/or oocyte donors verified the presence of P. pneumotropica and/or of antibodies to MHV in all strains, but neither in the recipients nor in the offspring antibodies to MHV could they be detected. The results indicate that an in vitro fertilization-embryo transfer (IVF-ET) system is an effective and simple alternative to cesarean operation in infected mice.<br>

Journal

  • Experimental Animals

    Experimental Animals 45 (1), 33-38, 1996

    Japanese Association for Laboratory Animal Science

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