Evidence for the Essential Role of Myosin Subfragment-2 in the ATP-Dependent Actin-Myosin Sliding in Muscle Contraction.
-
- TSUCHIYA Teizo
- Department of Biology, Faculty of Science, Kobe University
-
- TANAKA Hiromitsu
- Department of Physiology, School of Medicine, Teikyo University
-
- SHIRAKAWA Ibuki
- Department of Physiology, School of Medicine, Teikyo University
-
- KARR Trudy
- Department of Biology, Johns Hopkins University
-
- SUGI Haruo
- Department of Physiology, School of Medicine, Teikyo University
Bibliographic Information
- Other Title
-
- Evidence for the Essential Role of Myos
Search this article
Abstract
The role of myosin subfragment-2 (myosin S-2) in muscle contraction was studied by using an in vitro motility assay system in which the ATP-dependent sliding between myosin-coated polystyrene beads and actin filament arrays (actin cables) of giant algal cells were recorded under constant external loads provided with a centrifuge microscope. With antibody to myosin S-2 below 0.3 mg/ml, the maximum “isometric” force generated by myosin molecules on the bead decreased markedly, but the unloaded bead-sliding velocity along actin cables did not change appreciably, indicating a decrease in the number of myosin molecules interacting with actin scables. The antibody at 0.3-1.5 mg/ml decreased not only the maximum isometric force, but also the unloaded bead-sliding velocity in a dose-dependent manner. With the antibody at 1.5-3 mg/ml, the beads eventually stopped moving to remain attached to actin cables. These beads could be readily detached from actin cables with very small centrifugal forces, indicating very weak actin-myosin linkages. The antibody had no effect on rigor actin myosin linkages fromed before the antibody application. These results are consistent with the view that myosin S-2 plays an essential role in muscle contraction.
Journal
-
- The Japanese Journal of Physiology
-
The Japanese Journal of Physiology 48 (5), 383-387, 1998
THE PHYSIOLOGICAL SOCIETY OF JAPAN
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205042957056
-
- NII Article ID
- 10008305472
-
- NII Book ID
- AA00691224
-
- COI
- 1:CAS:528:DyaK1MXlvFWg
-
- ISSN
- 18811396
- 0021521X
-
- NDL BIB ID
- 4615131
-
- PubMed
- 9852347
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed