The Underlying Cellular Mechanism in the Effect of Tetramethylpyrazine on the Anion Secretion of Colonic Mucosa

DOI Web Site PubMed 参考文献32件
  • Zhao Wen-Chao
    Departments of Physiology, Medical School, Zhengzhou University
  • Duan Dong-Xiao
    Departments of Physiology, Medical School, Zhengzhou University
  • Wang Zhi-Ju
    Departments of Physiology, Medical School, Zhengzhou University
  • Tang Ning
    Pharmacy, Medical School, Zhengzhou University
  • Yan Ming
    Departments of Physiology, Medical School, Zhengzhou University
  • Zhang Gui-Hong
    Departments of Physiology, Medical School, Zhengzhou University
  • Xing Ying
    Departments of Physiology, Medical School, Zhengzhou University

書誌事項

タイトル別名
  • Underlying Cellular Mechanism in the Effect of Tetramethylpyrazine on the Anion Secretion of Colonic Mucosa

この論文をさがす

説明

The present study investigated the underlying cellular mechanism in the effect of ligustrazine (tetramethylpyrazine, TMP) on the anion secretion of colonic mucosa in rats using a short-circuit current (Isc) technique in conjunction with “tool drugs.” (i) After a pretreatment of the tissues by bathing the bilateral surface with Cl−-free Krebs-Henseleit (K-H) solution for over an hour, a basolateral application of 1 mmol/l TMP produced an increase in Isc, and the total charges transported for 30 min were about 8.7 ± 1.4 mC/cm2; an apical pretreatment of DPC and a basolateral addition of acetazolamide decreased the TMP-induced Isc by about 60% (P < 0.01) and 45% (P < 0.05), respectively; a basolateral application of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), the inhibitor of Na+-HCO3− cotransporter (NBC), did not alter the TMP-induced Isc. (ii) After the bilateral surface of mucosa was bathed with HCO3−-free K-H solution for over an hour, a basolateral application of 1 mmol/l TMP produced an increase in Isc, and the total charges transported in 30 min were about 8.3 ± 1.9 mC/cm2; an apical pretreatment of DPC (1 mmol/l), the inhibitor of Cl− channels, decreased the TMP-induced Isc by about 84% (P < 0.01). The basolateral presence of bumetanide (0.1 mmol/l), the inhibitor of Na+-K+-Cl− cotransporter (NKCC), significantly reduced the TMP-evoked Isc by about 86% (P < 0.01). In conclusion, (i) ligustrazine could promote colonic mucosa secretion Cl− via apical Cl− channels and basolateral NKCC; (ii) ligustrazine could promote colonic mucosa secretion HCO3− via apical Cl− channels and the basolateral diffusion of CO2.<br>

収録刊行物

参考文献 (32)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ