Effect of Bite-Opening and Cyclosporin A on the mRNA Levels of Myosin Heavy Chain and the Muscle Mass in Rat Masseter
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- Arai Chihiro
- Department of Orthodontics, Tsurumi University School of Dental Medicine
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- Ohnuki Yoshiki
- Department of Physiology, Tsurumi University School of Dental Medicine
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- Umeki Daisuke
- Department of Orthodontics, Tsurumi University School of Dental Medicine
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- Saeki Yasutake
- Department of Physiology, Tsurumi University School of Dental Medicine
書誌事項
- タイトル別名
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- Effects of Bite-Opening and Cyclosporin A on the mRNA Levels of Myosin Heavy Chain and the Muscle Mass in Rat Masseter
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To gain more insight into the mechanism of muscle plasticity in response to mechanical overload, we analyzed the effects of bite -opening (BO, 3 mm increase in the vertical dimension for 2 weeks) and/or a calcineurin (CaN) inhibitor, cyclosporin A (CsA, 10 mg/kg body weight, once daily for 2 weeks, ip) treatment on the myosin heavy chain (MHC I, IIa, IId/x, IIb) mRNA levels, using real-time RT-PCR with specific primers and on the muscle mass in rat masseter. As compared with normal control (n = 6), the BO treatment (n = 6) significantly increased the MHC I (p < 0.05) and the IIa mRNA levels (p < 0.01), and the CsA treatment (n = 6) significantly decreased the MHC I mRNA level (p < 0.01) in association with the significant decrease in the MHC IIb mRNA level (p < 0.05). The BO + CsA treatment (n = 6) significantly increased the MHC IIa mRNA level (p < 0.01) in association with the significant decrease in the MHC IIb mRNA level (p < 0.01), as compared with control. The masseter muscle mass was significantly decreased by either the CsA (p < 0.05) or the BO + CsA treatment (p < 0.001), but slightly increased by the BO treatment. These results suggest that in rat masseter the BO treatment produces not only the up-regulation of MHC IIa mRNA independently of CaN-signaling pathways, but also the MHC mRNA transition from IIa to I and the muscle mass maintenance mainly of type IIb fiber through the CaN-signaling pathways.<br>
収録刊行物
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 55 (3), 173-179, 2005
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205043673984
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- NII論文ID
- 10016882458
- 130004435928
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- NII書誌ID
- AA00691224
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- ISSN
- 18811396
- 0021521X
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- NDL書誌ID
- 7715789
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可