Transcriptional and Histological Analyses of the Thymic Developmental Process in the Fetal Pig
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- Suzuki Shunichi
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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- Suzuki Misae
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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- Nakai Michiko
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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- Sembon Shoichiro
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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- Fuchimoto Daiichiro
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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- Onishi Akira
- Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba,Ibaraki 305-0901, Japan
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Abstract
The humanized pig model, in which human cells or tissues can be functionally maintained in pigs, can be an invaluable tool for human medical research. Although the recent development of immunodeficient pigs has opened the door for the development of such a model, the efficient engraftment and differentiation of human cells may be difficult to achieve. The transplantation of human cells into fetal pigs, whose immune system is immature, will ameliorate this problem. Therefore, we examined the development of porcine fetal thymus, which is critical for the establishment of the immune system. We first analyzed the levels of mRNA expression of genes that are relevant to the function of thymic epithelial cells or thymocytes in whole thymi from 35 to 85 days of gestation (DG) and at 2 days postpartum (DP) by quantitative RT-PCR. In addition, immunohistochemical analyses of thymic epithelial cells from DG35 to DG55 and DP2 were performed. These analyses showed that the thymic cortex was formed as early as DG35, and thymic medulla gradually developed from DG45 to DG55. These findings suggested that, at least before DG45, the thymus do not differentiate to form fully functional T cells.
Journal
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- Experimental Animals
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Experimental Animals 63 (2), 215-225, 2014
Japanese Association for Laboratory Animal Science
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Details 詳細情報について
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- CRID
- 1390001205044046464
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- NII Article ID
- 130003391620
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- NII Book ID
- AA11032321
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- COI
- 1:STN:280:DC%2BC2cnntlyjtw%3D%3D
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- ISSN
- 18817122
- 00075124
- 13411357
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- NDL BIB ID
- 025412054
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- PubMed
- 24770647
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed