Effects of Cholesterol-Loaded Cyclodextrins on the Rate and the Quality of Motility in Frozen and Thawed Rabbit Sperm

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  • Nishijima Kazutoshi
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
  • Yamaguchi Shinji
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
  • Tanaka Mai
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
  • Sakai Yusuke
    Department of Bio-resources, Division of Biotechnology, Frontier Science Research Center, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
  • Koshimoto Chihiro
    Department of Bio-resources, Division of Biotechnology, Frontier Science Research Center, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
  • Morimoto Masatoshi
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
  • Watanabe Teruo
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan
  • Fan Jianglin
    Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan
  • Kitajima Shuji
    Division of Biological Resources and Development, Analytical Research Center for Experimental Sciences, Saga University, 5–1–1 Nabeshima, Saga 849-8501, Japan

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Abstract

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analysis system. Sperm motility with CLC supplementation was 29.4 ± 9.6% (mean ± SD), which was significantly higher than that of controls (20.8 ± 7.1%, P<0.05). The curvilinear velocity of sperm with CLC exceeded that of controls, whereas the values for linearity and wobble were significantly lower in sperm with CLC compared with controls. After artificial insemination, 44.3% of recovered ova were fertilized in the CLC-supplemented group, which was higher than the percentage in the control group (36.4%). The results indicate that supplementation with CLC improves the rate and quality of motility in rabbit sperm after freezing and thawing, and would be advantageous for successful cryopreservation.

Journal

  • Experimental Animals

    Experimental Animals 63 (2), 149-154, 2014

    Japanese Association for Laboratory Animal Science

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