Noninvasive Monitoring of beta-Cell Mass and Fetal beta-Cell Genesis in Mice Using Bioluminescence Imaging
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- Sekiguchi Yukari
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Owada Junya
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Oishi Hisashi
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Katsumata Tokio
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Ikeda Kaori
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Kudo Takashi
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
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- Takahashi Satoru
- Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba
書誌事項
- タイトル別名
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- Noninvasive Monitoring of β-Cell Mass and Fetal β-Cell Genesis in Mice Using Bioluminescence Imaging
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抄録
Bioluminescence imaging (BLI) has been applied in gene therapy and research to screen for transgene expression, progression of infection, tumor growth and metastasis, and transplantation. It enables real-time and relatively noninvasive localization and serial quantification of biological processes in experimental animals. In diabetes research, BLI has been employed for the quantification of β-cell mass, monitoring of islet graft survival after transplantation, and detection of reporter gene expression. Here, we explore the use of BLI in a transgenic mouse expressing luciferase under the control of the mouse insulin 1 promoter (MIP-Luc-VU). A previous report on MIP-Luc-VU mice showed luminescence intensities emitted from the islets correlated well with the number of islets in vitro and in vivo. In this study, we showed MIP-Luc-VU mice fed a high fat diet for 8 weeks gave rise to a greater bioluminescent signal than mice fed a regular diet for the same period of time. Conversely, there was a strong reduction in the signal observed in diabetic Mafa-deficient/Mafk-transgenic mutant mice and streptozotocin-treated mice, reflecting the loss of β-cells. Furthermore, we were able to monitor fetal β-cell genesis in MIP-Luc-VU mice during the late gestational stage in a noninvasive and repetitive manner. In summary, we show that bioluminescence imaging of mice expressing a β-cell specific reporter allows detection of changes in β-cell mass and visualization of fetal β-cell neogenesis in uteri.<br>
収録刊行物
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- Experimental Animals
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Experimental Animals 61 (4), 445-451, 2012
公益社団法人 日本実験動物学会
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詳細情報
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- CRID
- 1390001205044085248
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- NII論文ID
- 10030800588
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- NII書誌ID
- AA11032321
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- COI
- 1:STN:280:DC%2BC38fksFaltg%3D%3D
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- ISSN
- 18817122
- 00075124
- 13411357
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- HANDLE
- 2241/118074
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- NDL書誌ID
- 023813872
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- PubMed
- 22850644
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可