Evaluation of Commercial Kits for a Rapid Detection of <I>Escherichia coli</I> O157 in Stool

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  • 糞便からの大腸菌O157の迅速同定のための市販検出キットの評価
  • フンベン カラ ノ ダイチョウキン O157 ノ ジンソク ドウテイ ノ タメ
  • Evaluation of Commercial Kits for a Rapid Detection of Escherichia coli O157 in Stool

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Abstract

To introduce rapid detection of E. coli O157 in the stool without culturing, nine different commercial kits for detection of E. coli O157 were compared. VIP, Reveal, Path Stik, Quix, Now EH and EZ coli were found to detect E. coli O157 greater than 106 CFU/ml. TECRA and Novapath could detect more than 105 CFU/ml. VIDAS reacted positive with more than 104CFU/ml.<BR>TECRA, Novapath, Quix could detect 1: 4, 1: 4, 1: 9 dilution of stool specimen spiked with E. coli O157 respectively, but others could not detect with 1: 9 dilution of stool specimen.<BR>When stool specimens spiked with E. coli O157 were diluted ten times, centrifuged slightly and supernatants were tested, Path Stik, Quix and EZ coli could detect 107 CFU/ml. TECRA and Novapath could detect 106 CFU/ml, whereas VIDAS could detect 105 CFU/ml<BR>Quix is therefore one of the most suitable kit for detection of E. coli O157 in the stool at the bedside, although some non-specific reactions were observed. Path Stik is also convenient kit at bedside. VIDAS, although it requires special equipment, is the most sensitive kit for detection E. coli O157 and is suitable for detection of low levels of E. coli O157 in the clinical laboratory.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 72 (1), 40-44, 1998

    The Japanese Association for Infectious Diseases

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