Characterization of specimens obtained by different sampling methods for evaluation of periodontal bacteria
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- Okada Ayako
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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- Sogabe Kaoru
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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- Takeuchi Hiroaki
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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- Okamoto Masaaki
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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- Nomura Yoshiaki
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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- Hanada Nobuhiro
- Department of Translational Research, School of Dental Medicine, Tsurumi University
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説明
<p>Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease. The purpose of this study was to compare the effectiveness of sampling of saliva, supragingival and subgingival plaque for evaluation of periodontal bacteria. From each of 12 subjects, i) subgingival plaque was collected from the deepest pocket using a sterile paper point, ii) stimulated whole saliva was collected after chewing gum, and iii) supragingival plaque was collected using a tooth brush. These samples were sent to the medical examination laboratory for quantitative analysis of the counts of three periodontal bacterial species: Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. The proportions of these bacteria in subgingival plaque were higher than those in saliva or supragingival plaque, but lower in subgingival plaque than in saliva or supragingival plaque. In several cases, periodontal bacteria were below the levels of detection in subgingival plaque. We concluded that samples taken from subgingival plaque may be more useful for evaluating the proportion of periodontal bacteria in deep pockets than is the case for other samples. Therefore, for evaluation of periodontal bacteria, clinicians should consider the characteristics of the specimens obtained using different sampling methods.</p>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 59 (4), 491-498, 2017
日本大学歯学部