Monoclonal Antibodies to Herpes Simplex Viruses Antigens-Specificity and Utility in Rapid Serotyping of Clinical Isolates

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  • 単純ヘルペスウイルスの型特異モノクローナル抗体作製および臨床分離株型別への応用
  • タンジュン ヘルペス ウイルス ノ カタトクイ モノクローナル コウタイ サク

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Description

Sixteen hybridomas secreting antibodies to HSV-1 and 22 hybridomas serecting antibodies to HSV-2 were derived from fusion of SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with each respective virus. Four of the former 16 hybridomas and seven of the latter 22 hybridomas were subcloned and injected into pristane-primed mice to obtain high titers of monoclonal antibodies. Antigen specificity of these monoclonal antibodies were determined by the Western blotting (WB) asaay. Two out of four monoclonal antibodies that showed selective reactivity for HSV-1 in IFA, reacted with HSV-1 specific proteins:#1 reacted with 100 KD and 70 KD proteins and #4 with a 150 KD protein, respectively, while the remaining two antibodies reacted only with a 50 KD protein that is type-common antigen. On the other hand, two out of seven antibodies which showed selective reactivity for HSV-2 in IFA, reacted with HSV-2 specific proteins:#5 with a 100 KD protein and #10 with three proteins of 30, 25, and 20 KD, and the other two antibodies reacted with a 50 KD protein that is a type-common antigen. The remaining three antibodies, two of which were found to be immunoglobulin type IgM, reacted with neither HSV-1 nor HSV-2 antigens in WB assay.<BR>In order to determine their utility in serotyping, 11 monoclonal antibodies were examined by IFA test for reactivity to cells that were infected with 20 HSV-1 or 16 HSV-2 isolates which had been typed by neutralization test. All of the antibodies except two showed the same specificity as demonstrated in the neutralization test. The two monoclonal antibodies, #5 and #10, which specifically reacted with HSV-2 antigens in WB assay, did not react with two and four isolates of HSV-2, respectively. Possible subtyping of HSV-2 using monoclonal antibodies was discussed.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 65 (3), 293-298, 1991

    The Japanese Association for Infectious Diseases

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